Project
Part:BBa_K153003:Design
Designed by: Dmitry O. Malyshev Group: iGEM08_University_of_Sheffield (2008-10-29)
CsrB without promoter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 48
Illegal SpeI site found at 581
Illegal PstI site found at 567 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 48
Illegal SpeI site found at 581
Illegal PstI site found at 567
Illegal NotI site found at 572 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 48
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 48
Illegal SpeI site found at 581
Illegal PstI site found at 567 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 48
Illegal SpeI site found at 581
Illegal PstI site found at 567 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 497
Design Notes
In the natural E.Coli system CsrA is an important gene, the product of which inhibits many genes responsible for glycogen formation, gluconeogenesis, and biofilm formation. During a quorum sensing event, it is in turn inhibited by CsrB, thus allowing biofilm formation.
Source
to be added