Project

Part:BBa_K153003:Design

Designed by: Dmitry O. Malyshev   Group: iGEM08_University_of_Sheffield   (2008-10-29)

CsrB without promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 48
    Illegal SpeI site found at 581
    Illegal PstI site found at 567
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 48
    Illegal SpeI site found at 581
    Illegal PstI site found at 567
    Illegal NotI site found at 572
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 48
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 48
    Illegal SpeI site found at 581
    Illegal PstI site found at 567
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 48
    Illegal SpeI site found at 581
    Illegal PstI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 497


Design Notes

In the natural E.Coli system CsrA is an important gene, the product of which inhibits many genes responsible for glycogen formation, gluconeogenesis, and biofilm formation. During a quorum sensing event, it is in turn inhibited by CsrB, thus allowing biofilm formation.

Source

to be added

References